目的 建立可用于中药远志质量控制的6种糖酯类成分的一测多评方法。方法 以3,6′-二芥子酰基蔗糖 (3,6′-disinapoyl sucrose, DISS) 为内参物,计算远志糖酯sibiricose A5、sibiricose A6、tenuifoliside B、tenuifoliside A、tenuifoliside C的相对校正因子,并进行含量计算,实现一测多评。同时实测20批远志药材,将校正因子法计算的含量值与外标法实测值进行比较,验证一测多评法的准确性。结果 Sibiricose A5、sibiricose A6、tenuifoliside B、tenuifoliside A、tenuifoliside C对DISS的相对校正因子分别为1.23、1.16、1.56、1.79、0.92,在不同的实验条件下重现性良好,一测多评法与外标法实测值间无显著差异。结论 一测多评法可用于远志药材中6种糖酯类成分的含量测定,方法成本低、准确、简便可行。
Abstract
OBJECTIVE To develop a method of quantitative analysis of multicomponents by singlemarker (QAMS) for simultaneous determination of six oligosaccharide esters in Polygalae Radix. METHODS 3,6′-Disinapoyl sucrose (DISS) was used as the internal reference substance. The relative correlation factors (RCF) of sibiricose A5, sibiricose A6, tenuifoliside B, tenuifoliside A, and tenuifoliside C to DISS were calculated. The contents of the six oligosaccharide esters in 20 different batches of Polygalae Radix were determined by both external standard method and QAMS. The assay results were compared to evaluate the accuracy of QAMS method. RESULTS The RCF of sibiricose A5, sibiricose A6, tenuifoiside B, tenuifoliside A, and tenuifoiside C to DISS were 1.23, 1.16, 1.56, 1.79, and 0.92, respectively. Under different experimental conditions, the RCF had good reproducibility, and there was no significant difference in the quantitative analysis results between the external standard method and QAMS method. CONCLUSION The QAMS method is feasible and credible, and can be used for the quality control of oligosaccharide esters in Polygalae Radix.
关键词
远志 /
糖酯 /
一测多评法 /
相对校正因子
{{custom_keyword}} /
Key words
Polygalae Radix /
oligosaccharide ester /
quantitative analysis of multi-components by single marker /
relative correlation factor
{{custom_keyword}} /
中图分类号：
R284
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Ch.P (2010) VolⅠ(中国药典2010年版. 一部). 2010:146-147.
[2] Jiangsu Institute of Botany. Outline of Xinhua Materia Medica VolⅠ(新华本草纲要. 一卷) . Shanghai:Shanghai Science and Technology Publishing House, 1988:292.
[3] SHEN J, ZHANG Q, PAN N, et al. Traditional Chinese medicine in the treatment of patients with anxiety-depression . Acta Acad Med Qingdao Univ (青岛大学医学院学报),2009, 45(2):113-114.
[4] LIU Y F, LI E Z. An analysis of antidepressant application in our hospital from year 2001 to 2002 . Med J Qilu (齐鲁医学杂志), 2004,19(4):343-344.
[5] TU H, LIU P, LIU Y, et al. Study on antidepressant components of sucrose ester from Polygala tenuifolia. China J Chin Mater Med (中国中药杂志),2008,33(11):1278-1280.
[6] LIU P, HU Y, GUO D H, et al. Antioxidant activity of oligosaccharide ester extracted from Polygala tenuifolia roots in senescence-accelerated mice . Pharm Biol, 2010,48(7):828-833.
[7] IKEYA Y, TAKEDA S, TUNAKAWA M, et al. Cognitive improving and cerebral protective effects of acylated oligosaccharides in Polygala tenuifolia. Biol Pharm Bull, 2004, 27 (7):1081-1085.
[8] LIU P, HU Y, GUO D H, et al. Potential antidepressant properties of Radix Polygalae (Yuan Zhi) . Phytomedicine, 2010, 17(10):794-799.
[9] ZHANG L Q, FENG L, NIE J. Overview and features of Chinese medicine quality standards . Chin Pharm J(中国药学杂志), 2010,45(17):1284-1286.
WANG Z T. Recent Advances and a prospect on the future development of quality assessment of traditional Chinese medicines . Chin J Nat Med (中国天然药物), 2006, 4(6):403-410.
WANG Z M, GAO H M, FU X T, et al. Multi-components quantitation by one marker new method for quality evaluation of Chinese herbal medicine . China J Chin Mater Med (中国中药杂志), 2006, 31(23):1925-1928.
WANG Z M, QIAN Z Z, ZHANG Q W, et al. A guidance to establisha method of quantitative analysis of multi-components by single marker . China J Chin Mater Med(中国中药杂志), 2011, 36(6):657-658.
Ch. P (2010) VolⅠ(中国药典2010年版. 一部). 2010:285-286.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
PDF(979 KB)
